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Plates were incubated for 15 min in a Spark 20M (Tecan) plate reader at 30 C and A 340nm was measured every 26 s with a 5 s shaking interval between measurements at 180 rpm using a 3 mm shaking diameter

The discovery trajectory of orforglipron, from its origins at Chugai Pharmaceutical in Japan to global Phase 3 development by Eli Lilly, illustrates how persistent scientific innovation can overcome what was once considered a pharmacological impossibility

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