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This dual-enzyme cascade is widely present in the cytoplasm and is highly sensitive to oxidative stress

2007;292(2):H1095-1104

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We reasoned that as the GluC cleavage specificity is different from the three catalytic activities of the proteasome, considering that the proteasome caspase-like site cleaves more efficiently after aspartic acid residues than glutamic acid (Kisselev et al, 2003), we could distinguish proteasome-generated peptides from those produced by GluC