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To mitigate miscellaneous bias and ensure reliable results, the PSM method was employed to adjust for differences in BMI and the rate of D3 top-quality embryos between the groups

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Plasma samples (20 l) were mixed with 500 L of 42 mM H2SO4, 125 L of phosphotungstic acid solution was added, and each sample was mixed by vortexing and incubated at room temperature for 5 minutes