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This review systematically categorizes biomarkers by pathogenic mechanism (neural conduction deficits, oxidative stress, inflammation, neurovascular injury, metabolic dysregulation, and genetic factors) to provide clinicians with a framework for interpreting complex DPN pathophysiology (Figure 5)

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The enzymatic recycling assay (DTNB/Ellmans method) is the most widely adopted technique for total glutathione measurement due to its accessibility and reproducibility

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