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Surface receptor probe was cleaved by incubation in 100 mM 2-mercaptoethane-sulfonic acid sodium salt (Mesna) in 1X TAE buffer, pH 8.6 or incubated 1X TAE buffer alone for 5 minutes and then washed and imaged in HBSS using a custom-written high content analysis software in Micro-Manager in a Cairn Research widefield microscope with Nikon Ti2, CoolLED light source and 20X phase contrast objective for both epifluorescence and transmitted phase contrast with 9 ROI acquired per well

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doi: 10.3390/molecules25030609 5

Approved in early 2026 by Novo Nordisk, it is the first oral GLP-1 approved specifically for weight loss, distinct from Rybelsus, which targets type 2 diabetes

[DOI] [PMC free article] [PubMed] [Google Scholar] 186.Montero Vega M.T., de Andrs Martn A

A., & Hussain, K