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Abbreviations OXPHOS: Oxidative phosphorylation mtDNA: Mitochondrial genome ROS: Reactive oxygen species DAMP: Damage-associated molecular pattern AMP: Antimicrobial peptide m: Mitochondrial membrane potential CCCP: Carbonyl cyanide 3- chlorophenylhydrazone AEL: After egg lay PCA: Principal component analysis CPM: Counts per million GO: Gene Ontology UPR mt : Mitochondrial unfolded protein response H 2 0 2 : Hydrogen peroxide NAC: N-acetylcysteine NADH: Reduced form of nicotinamide adenine dinucleotide ETC: Electron transport chain MAPK: Mitogen-activated protein kinase RNAi: RNA interference VDRC: Vienna Drosophila Resource Center TMRM: Tetramethylrhodamine methyl ester RT-qPCR: Reverse Transcription Quantitative Real-time PCR RT: Reverse transcriptase Ct: Threshold cycle BSA: Bovine Serum Album PBS: Phosphate Buffered Saline FDR: False discovery rate DMSO: Dimethyl Sulfoxide PI: Propidium iodide References Monzio Compagnoni G, Di Fonzo A, Corti S, Comi GP, Bresolin N, Masliah E

Most home refrigerators experience door-opening temperature spikes of 24C multiple times per day, and few users track cumulative exposure time above threshold

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Professionals use single-use, medical-grade equipment to eliminate the risk of contamination virtually

& Caroni, P