At 4-months-old, GKHCD rats were randomly assigned to three groups (N = 6): no intervention (GKHCD), sleeve gastrectomy (GKHCD_SL), and sham surgery (GKHCD_Sh)
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In this model, iodine deficiency and Se deficiency exacerbate H2O2 accumulation, Se deficiency decreases cellular defense, promotes thyroid cell fibrosis, increases the inflammatory response following thyroid cell necrosis, and thiocyanate overload triggers follicular cell necrosis
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The cells were seeded at an appropriate density in 6-well plates and divided into three experimental groups: NC (normoxia control), HY (hypobaric hypoxia), and hypoxia combined with KW6002 treatment groups (HY + 4 M, HY + 2 M, HY + 1 M, HY + 0.5 M, HY + 0.25 M)