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doi: 10.3389/fnut.2022.900421 Received 20 March 2022 Accepted 12 April 2022 Published 06 May 2022 Volume 9 - 2022 Edited by Fuguo Liu, Northwest A&F University, China Reviewed by Ziwei Zhang, Northeast Agricultural University, China

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For mKate reporter construction, the mKate2 gene was amplified from pRN10 (Addgene, plasmid 84454) 69 using a specific primer pair (Supplementary Table 3) and Q5 High-Fidelity DNA Polymerase (New England Biolabs)

doi: 10.1016/j.carbpol.2024.122768 194 LiangDFengYZandkarimiFWangHZhangZKimJet al

The 50:10:10:10 ratio ensures effective metal-ligand complexation across the reconstituted volume and reflects the compositional standard documented in published KLOW formulation literature