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DAB staining and preparation of cultured cells for EM The fixative was removed and cells were washed (five times for 1 min) with 0.1 M sodium cacodylate (Ted Pella, 18851) buffer at pH 7.4 containing 2 mM CaCl 2 on ice and blocked with 20 mM glycine in 0.1 M sodium cacodylate buffer pH 7.4 containing 2 mM CaCl 2 for 20 min on ice

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Three basal measurements of OCR and ECAR were measured over 24 min of three repeats of mix (3 min)/wait (2 min)/measurement (3 min) using the Seahorse XFe24 analyser

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Summary: Ozempic can cause itching, most commonly as mild, localised injection site reactions affecting 110% of patients, with generalised pruritus or urticaria occurring less frequently in 0.11% of patients

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