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This prevents peptide from tracking back through the needle path

The following day, Ni-NTA resin equilibrated in buffer A was added over the cleaved laccase samples and incubated at 25 C and 1,000 rpm in a thermomixer comfort shaker (Eppendorf) for 4 h

Purity verification for a laboratory grade peptide like GHK-Cu should rely on supplier-provided Certificates of Analysis that include HPLC chromatograms, purity percentage (typically expressed as 98% for research-grade material), and mass spectrometry data confirming molecular identity

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