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Subsequently, the cells were stained with the Hoechst 33342 nuclear dye (Thermo Fisher Scientific, Inc.) at a 1:500 dilution in PBS at room temperature for 10 min, washed with PBS, and visualized using a BZ-X700 (Keyence Corporation)

Certain genetic disorders can cause true neurodegenerative dementia in younger populations (e.g

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doi: 10.1111/j.1365-2125.1980.tb01820.x