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Then, cells were placed in a selective medium containing hygromycin B (100 g/mL for MCF-7 and 200 g/mL for SUM-159 and MDA-MB-231)

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The latter is superior to the oral route since glutathione passes directly into systemic circulation resulting in a much higher rate of absorption compared to that achieved by oral intake

Store it in a temperature-controlled spot to keep it fresh