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Heme-Rbt and heme-HuHPX Affi-Gel 15 affinity-isolated proteins were fractionated using SDS-PAGE electrophoresis, transferred to PVDF membranes, and biotinylated proteins detected using streptavidinHRP

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The funders had no role in the design of the study

Clin Sci (Lond) 133(20):20452059

suggested a difference in the individual MTHFR gene variation ( In contrast to the aforementioned study ( Importantly, we did not preselect patients based on a positive response to previous vitamin B12 injections, leading to more generalizable results in our study