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Mounted sections were next incubated for 90 min in cytomailers with buffer containing 10 mM MgCl 2 , 0.1% bovine serum albumin and 100 pM [ 125 I] OVT (NEX2540, PerkinElmer, Boston MA)

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Step 1: Aseptic Reconstitution Before you even touch a syringe, you must properly prepare your peptide