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Semi-quantitative analysis was performed

Use ice early and heat later : apply ice during the first 2448 hours to reduce inflammation, then switch to heat to relax muscles and improve blood flow

The probe was removed, fresh PBS was added and the fluorescence was immediately read using a FLx800 Fluorescence microplate reader (Mason Technology)

These commenters stated that CMS should delay the removal of the measures so as to not erode the substantial efforts already underway

doi: 10.3892/ijo.2014.2597