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(2) V3-V4 Amplification Target regions were amplified with barcoded primers 341 F (5-CCTAYGGGRBGCASCAG-3) and 806 R (5-GGACTACNNGGGTATCTAAT-3) using Phusion High-Fidelity PCR Master Mix (New England Biolabs, USA)
59% were males
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Metformin has also been shown to improve insulin secretion from rat islets chronically exposed to high levels of NEFA or glucose [21], suggesting a direct effect on pancreatic beta cells independent of its glucose-lowering action
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