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Formula Dosage (mcg) = (Vial mg Total ml) Injection volume (ml) 1000 Example Calculation Vial: 5 mg GHK-Cu Water added: 2 ml Injection volume: 0.1 ml (5 mg 2 ml) = 2.5 mg/ml 2.5 mg/ml 0.1 ml = 0.25 mg 0.25 mg 1000 = 250 mcg Therefore, 0.1 ml equals 250 mcg

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For the control group, after the same initial treatment, the FOXO4-DRI was replaced with an equal volume of PBS

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