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Methionine is an amino acid that acts as a lipotropic agent to prevent excess fat buildup in the liver and the body

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Bring to room temperature let the vial equilibrate ~1520 min to avoid condensation

This procedure involved removing the supernatant and resuspending the mitochondrial pellet in an isolation buffer of 250 mM sucrose, 0.5 mM EDTA, and 10 mM Tris-HCl to achieve a final protein concentration of 20-25 mg/ml [2]