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Knocking out FDX1 in cancer cells leads to a complete loss of protein lipoylation, highlighting its central role in this pathway 15

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Wilhelm S, et al

Physical Agitation: While not directly temperature-related, it's worth noting that vigorous shaking can also physically shear the peptide chains apart

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Beaven SW, Matveyenko A, Wroblewski K et al (2013) Reciprocal regulation of hepatic and adipose lipogenesis by liver X receptors in obesity and insulin resistance