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We utilized a novel surgical procedure in diet-induced obese (DIO)(Flynn et al., 2015) and lean(Albaugh et al., 2019) mice wherein bile is diverted from the gallbladder (GB) to the duodenum (GB-D, a sham procedure), the jejunum (GB-J), or ileum (GB-IL)

Z.et al (2018)

The weight loss comes from the deficit, not from the peptide directly burning calories, except for very modest thermogenic effects

Avoid taking it too close to bedtime, as increased energy might interfere with sleep for some

LOT2D, later-onset type 2 diabetes

no single diet mandated per ADA 2026 Physical activity: 150 min/week moderate-intensity aerobic activity