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Pharmacokinetic parameters after intravenous administration in rats showed Dihexa possessed a long half-life of 12.68 days and was extensively distributed, indicated by a large volume of distribution (Va)

In organotypic hippocampal slice cultures, Dihexa and Nle-AngIV similarly augmented spinogenesis, with control slices showing 7 spines per 50-m dendrite length, compared to 11 for both Dihexa and Nle-AngIV treated slices [1]

Cells were fixed with 4% PFA for 30 minutes, permeabilized with 0.1% Triton X-100, and blocked with 5% normal goat or donkey serum

At two weeks post-surgery all 10 of the experimental groups had grades ranging from 2.3 to 4.00, however, motor function ended up deteriorating by week 16 post-surgery

The overarching material developed and investigated comprises AngIV-based compounds, designed to overcome the limitations of native peptides like AngIV, which suffer from poor metabolic stability and inability to cross the BBB due to their size and hydrophilicity

Dihexas ability to drive spinogenesis allows researchers to study structural neural regeneration and the restoration of lost synaptic connections in damaged brain tissue models