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The immediate post-discontinuation period (days 1-14) typically shows minimal visible changes as existing improvements from recent GHK-Cu application persist through maintained dermal copper levels, ongoing collagen synthesis from recently stimulated fibroblasts, and continued anti-inflammatory effects from treatment-induced changes

For example, antimicrobial sulfa drugs are used in urinary tract infections, ear infections and to some extent in malaria as well

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Increases synaptic proteins (synaptophysin, PSD-95) supporting connections between retinal neurons and from retina to brain

The articles discussion of collagen biology and preclinical mechanism relies on this research lane to distinguish cellular and tissue-model findings from broader human therapeutic claims

The injection itself is nearly painless due to the small gauge and shallow depth