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10.1016/j.mce.2022.111783 26 DrobnisE

6a), in most of the diseases a correlation was observed

In rodents, acute toxicity studies indicate that curcumin dosages exceeding 2000 mg/kg may modify liver enzymes, implying possible hepatic stress (Sirasanagandla et al., 2022)

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Samples were then incubated with primary antibodies in blocking buffer overnight at 4 C, washed three times with PBTA, then incubated with secondary antibody in blocking buffer for 1 h at room temperature