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Isopropyl--D-thiogalactopyranoside (IPTG) was added to the final concentration of 0.4 mM to induce the expression of recombinant protein at 25C for at least 5 h

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Compared with non-genetically modified plants, folate content increased 0.17150-fold in rice grains, 225-fold in tomato, 212-fold in potato tubers, 2.3-fold in wheat grains, 2.18.5-fold in lettuce, 24.2-fold in maize grains, 3-fold in Mexican common bean, and 1.34-fold in Arabidopsis leaves after genetic modification 532,752,754,791,803,804,805,806,807,808,809,810,811,812

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