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Effective systemic dose in pigs of 1.1 mg/kg (~75 mg in humans) showed no adverse effects, representing approximately 300-fold below toxic dose

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An open trial that included 32 women with PCOS found a multi-nutrient supplement providing 400 mcg (680 mcg DFE) 5-MTHF daily for three months decreased homocysteine levels compared with no treatment

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1-3Sulforaphane reduces intracellular survival of Staphylococcus aureus in macrophages through inhibition of JNK and p38 MAPK-induced inflammation