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Injection frequency gets debated at length

Lancet 2010;375:318-28

JCI Insight 5 , e122312 (2020)

Incubations were terminated by addition of ice-cold perchloric acid at a final concentration of 1% and plates were kept on ice for 10 min

Biofortification, i.e., the enhancement of natural folate content in plants, holds the potential to reach the required folate levels, which are low, particularly in staple crops, such as rice, potato, maize, wheat, and cassava 752,753,754,755

Geraghty RJ, Capes-Davis A, Davis JM, Downward J, Freshney RI, Knezevic I, Lovell-Badge R, Masters JR, Meredith J, Stacey GN, Thraves P, Vias M, Cancer Research UK (2014) Guidelines for the use of cell lines in biomedical research