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2.9 Assessment of oxidative stress markers GSH levels were assessed using kits from Biodiagnostics, Giza, Egypt, following a previously reported method ( Moreover, the antioxidant CAT enzyme level was determined using kits purchased from Biodiagnostics, Giza, Egypt

When butyrate was administered directly to intestinal epithelial stem cells, which are located at the base of the crypts and are anatomically shielded from exposure to SCFAs, it inhibited HDAC activity and increased H3K9 and K27 acetylation, which was accompanied by decreased proliferation and a diminished ability to respond to injury [147, 148]

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Despite their conclusions, the researchers warned that their findings should be interpreted with caution, and stressed that they have not proven a causal connection between GLP-1s, mortality or cognition

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