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The GSH levels of the BSO-treated cells quantified using our RT-based flow cytometry method is well correlated to the values measured using cell lysates with a glutathione reductase-based biochemical assay ( R 2 =0.99, Fig

Because several of these compounds are sensitive to heat and light, careful storage is part of getting reproducible results a degraded compound behaves differently from a fresh one

Fer-1 increased viability to 90% of cells under Cys 2 starvation and in BSO-treated cells, and to 70% in erastin-treated cells (Fig

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However, during apoptosis, the mitochondrial membrane potential is lost and the mitochondrial permeability transition pore (MPTP) remains open, allowing TMRE to escape into the cytoplasm, resulting in a significant reduction of orange-red fluorescence within the mitochondria [44]