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This delayed passage of food through the digestive system can lead to harder, less frequent stools, particularly during dose escalation or in patients with pre-existing bowel concerns

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For cell samples, BA and hepatocytes were lysed in lysis buffer (10 mM Tris pH 7.5, 5 mM EDTA, 50 mM HCl, 30 M sodium pyrophosphate, 50 mM NaF, 100 mM sodium o-vanadate, 1% Triton X-100, 1 mM PMSF and 10 g/mL protease inhibitors), pH 7.47.6

Gastric mucosal cells produce proglucagon, GLP-1, and GLP-2 (Ribeiro-Parenti et al., 2021)

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