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Potential mechanism of opioid peptides G protein-coupled receptors (GPCRs) are one of the four major classes of receptors embedded within the cell membrane, alongside ion channels, tyrosine kinase-coupled receptors, and intracellular receptors
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At the end point, cells were washed 3 times with saline and stained for 20 min at room temperature using a saline solution containing 0.2 mM l-glutamic acid -(4-methoxy--naphthylamide) (GMNA