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Cells were serially diluted and were plated onto rich or minimal medium containing the desired concentration of gliotoxin, using a multi-pronged replicator

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For the FOXO4-DRI treatment group, 50 M FOXO4-DRI (MCE, HY-P4157) was added to the glucose-free DMEM (Gibco, Catalog #11966-025) medium, followed by 3 h of oxygen-glucose deprivation (OGD) treatment

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