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Hogg E, Wu T, Bresee C, Wertheimer J, Malatt C, Tan E, et al

For protein purification, the cell pellet was resuspended in 120 mL of the lysis buffer (50 mM Na 2 HPO 4 , pH 8.0, 500 mM NaCl and 30 mM imidazole), and affinity chromatography was performed using a 35 mL Omnifit glass column packed with Ni Sepharose 6 Fast Flow (Cytiva)

Teerasarntipan, T., Chaiteerakij, R., Prueksapanich, P., & Werawatganon, D

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