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In 2022, another meta-analysis included 98 patients that had advanced HF and a BMI of at least 35 kg/m2, that underwent bariatric surgery [76]

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Briefly, PLA probes (anti-mouse PLUS and anti-rabbit MINUS) were diluted 1:5 in Duolink antibody diluent and incubated to detect anti-HA (rabbit) and anti-biotin (mouse) antibodies for 1 h at 37 C

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