Following dehydration in ascending concentrations of ethanol solutions (5 min washes in 50%, 70%, 100%, and 100% ethanol), slides were air-dried, and a hydrophobic barrier was created around the sections using a hydrophobic pen (Vector Labs)
If weight falls but BFM is stable, water or muscle, not fat, is driving the scale change
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The results of a TAC assay should be carefully interpreted in the specific clinical context, and possibly, together with others parameters of oxidative damage (e.g., a SOD assay activity) and hormone quantification (e.g., dehydroepiandrosterone and testosterone), as hormones seem to influence antioxidants levels [73]
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