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In order to test whether Epitalon has a protective effect on spindle integrity in oocytes, we added 0.1mM Epitalon to the culture medium to observe spindle integrity at 6h, 12h, and 24h of culture

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Although instructions for patients are supplied with individual bottles, procedures for use should be demonstrated to each patient.

For negative controls, primers were directed at the m6A-negative region of SLC7A5, and for positive controls, they were directed at the m6A-positive region [23]

Since D1-3 are selenoproteins, selenium deficiency would be expected to result in their reduced activities in different tissues, although this effect is observed only for D1 in the liver and kidney and not for D2 and D3 in other tissues (48)

Discard any vial where you suspect sterile technique was compromised during reconstitution