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As a peptide substance, it is highly sensitive to temperature, humidity, and proteases in the gastrointestinal tract

Since most reactions in the second stage are spontaneous, tyrosinase acts as the rate-limiting enzyme in melanin synthesis, making it the primary enzyme involved in both enzymatic browning and melanin production 7

Prior to analysis, the XFe96 instrument (Seahorse Biosciences, North Billerica, MA, USA) mixed the assay media in each well for 10 minutes to allow the oxygen partial pressure to reach equilibrium

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SeChry was chosen as a plausible competitive inhibitor of xCT as xCT is also able to take up selenium

Life Sci 2014