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20 mM alkylation buffer (from 1 M iodoacetamide in water) was added and kept at RT for 45 min in the dark

Furthermore, the use of ABPP to profile proteomic states has largely been restricted to lysates 8,10 , where protein interactions and compartmentalization are disrupted, potentially obscuring PTM-regulated binding events or generating artifacts due to probing of non-native conditions

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