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Statistical analysis for animal studies Statistical analyses were performed using GraphPad Prism v.8.4.3

injections of 100 nmol kg 1 GLP-1MK-801 ( n = 8 mice and n = 4 cages), 300 nmol kg 1 GLP-1MK-801 ( n = 8 mice and n = 4 cages) or vehicle (isotonic saline, n = 8 mice and n = 4 cages) for 14 days

During a drug holiday specifically, sleep quality becomes even more important because you have lost the medication-driven appetite suppression that previously compensated for suboptimal sleep habits