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Its a natural hormone your body makes in the gut after eating

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The engineered commensal bacteria were cultured at 1% (v/v) in de Man, Rogosa and Sharpe broth (Difco, VWR, Philadelphia, PA, USA) for ~17 h at 37C under anerobic conditions [anerobic jars with Anaerocult A Gas Packs (Merck, Darmstadt, Germany)] until stationary phase and centrifuged (16,900 g for 15 min, at 4C

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And lastly, I think its the uneven distribution that we talked about, especially with the chemotherapy drugs