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TUNEL assay On experimental day P38, pancreas samples were fixed in 2% w/v paraformaldehyde for 2 h and processed for TUNEL assay, which was performed using the One Step TUNEL Apoptosis Assay Kit (Beyotime, Shanghai, China) according to the manufacturers instructions

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In elderly patients, it is contraindicated if the estimated glomerular filtration rate is less than 60 ml/min/1.73 m 2

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