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Statistical analysis Differences between groups of MSCs were analyzed using Students t-test or one-way analysis of variance

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Beta blockers can be taken concurrently with retatrutide, but they may not fully control the heart rate increase because retatrutide works through the glucagon receptor cAMP-PKA pathway rather than the sympathetic nervous system that beta blockers target

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In each sample, GAPDH was used as an internal reference, and differences in total RNA were normalized