Add 5 mL of 1 mol/L potassium hydroxide solution and vortex to mix
Possibly, an appropriate dosage may prove to rely on individual characteristics
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Insert the needle at a 45-to-90-degree angle depending on subcutaneous fat depth
Furthermore, intestinal I/R injury can activate the vagus nerve, transmitting signals to the brain that influence neuronal activity and cognitive function, and alter gut microbial metabolite production, such as short-chain fatty acids (SCFAs), thus affecting neuronal signaling and cognitive processes (Wu et al., 2021
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