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2.10 Immunofluorescence assay Cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.5% Triton X-100 for 20 minutes, and blocked with 10% goat serum for 30 minutes

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Lysosomes Membrane-bound organelle containing digestive, hydrolytic enzymes whose function is primarily the degradation of macromolecules, old cell parts and microorganisms