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FIGURE 6 Anwuligan promotes HFSC proliferation In this study, CCK-8, MTT, and Edu assays were utilized to investigate the effect of anwuligan on the biological properties of HFSCs

Surgery and spinal cord injury Deeply anesthetized (3% isoflurane, ketamine 50 mg/kg b.w.) rats were subjected to laminectomy at lumbar level L2L3, which corresponds to the sacrocaudal spinal cord (S2-Co1) as described previously [42]

Additionally, NLRP3 is an important protein in mediating cell pyroptosis

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Then, these cells were treated with different concentrations of SP (cells/mL, 6.2 10 5 , 1.2 10 6 , 2.5 10 6 , 5 10 6 , 1 10 7 ) for 24 h

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