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Mar 2015;103(3):749-55.e2

Copper complexation is performed by mixing the purified tripeptide with copper(II) salts (chloride, acetate, or sulfate, depending on the protocol) at controlled pH, with the resulting complex purified by chromatography

Mirza AU, Kareem A, Nami SAA, Khan MS, Rehman S, Bhat SA, et al

Stacking with intention does not create evidence A phased protocol can sound scientific while still lacking validated dosing, interaction studies, comparative outcomes, or long-term safety

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Later, it gained popularity in the fitness community due to its potential to enhance tissue recovery and performance