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In vitro kinase assay Recombinant PRMT7 and AKT1 proteins were incubated together in kinase buffer (50 mM Tris-HCl, pH 7.5, 10 mM MgCl, 1 mM DTT) in the presence or absence of 1 mM ATP at 30 C for 30 min

Laboratory Safety Protocols General chemical safety practices should always be followed when handling research compounds

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Any estimated increase in relative risk must be interpreted in the context of the low overall incidence of NAION

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A single 12-hour room-temperature exposure is enough to denature 4060% of the peptide content in a reconstituted vial