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Samples in extraction mix were vortexed for 10 min at 4 C and centrifuged at 15,000 rpm for 10 min at 4 C to pellet insoluble material

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(nicotinamide adenine dinucleotide) NAD+ is a vital coenzyme essential for everything from energy metabolism to DNA repair

Nonetheless the TMD conformation differed and, overall, the receptor was relatively stable, exhibiting less flexibility compared with GLP-1R bound to exendin-4 or oxyntomodulin (Supplemental Figure 12)

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