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Briefly, brains of 3MST knockout mice or the wild-type mice were removed at the postnatal day 1 or 2 and submerged in the ice-cold Leiboritzs L-15 medium (Life Technologies, Waltham Massachusetts, USA)

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We schedule follow-up consultations to assess progress and adjust prescriptions as needed

Lyophilized: Hold the dry vial at 20 C (4 F) or colder in dry, dark conditions for up to 12 months [7]