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The forward primer was (5-GCGGCCGCCAGCTT TCTAGA atggctgggaaaccgaag-3, the underlined was XbaI (Vazyme Biotech Co., Ltd., Nanjing, China) site), and the reverse primer was (5-GAGATGAGTTTTTGT TCTAGA tta ATGATGATGATGATGATG gaaactgaatatttt-3, the underlined was XbaI site, the italic was 6 His tag)
Cell Cycle , 20 (18), 17601784
Genetic Evidence that Small Maf Proteins Are Essential for the Activation of Antioxidant Response Element-dependent Genes
Secondly, the NVT (T = 298 K) and NPT (P = 1.0 bar) ensemble equilibration were performed to stabilize the systems through the velocity rescaling thermostat and Berendsen barostat, respectively
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