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While ad libitum (AL)-fed mice eat in multiple small bouts throughout the day, with most of the intake during the night, CR regimens are performed by feeding mice only once-per-day
The difference lies in pulsatile GH release patterns
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Dosing syringe Use U-100 insulin syringes (29-31G, 0.3 mL or 0.5 mL) for measuring doses
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